ABSTRACT
Background: Adiponectin, an adipocyte-secreted hormone, is known to have anti-atherogenic, anti-inflammatory, and anti-diabetic properties. In the present study, the association between two common single nucleotide polymorphisms [SNPs] [+45T/G and +276G/T] of ADIOPQ gene and coronary artery disease [CAD] was assessed in the subjects with type 2 diabetes [T2DM]
Methods: Genotypes of two SNPs were determined by polymerase chain reaction-restriction fragment length polymorphism in 200 subjects with T2DM [100 subjects with CAD and 100 without CAD]
Results: The frequency of TT genotype of +276G/T was significantly elevated in CAD compared to controls [X2=7.967, P=0.019]. A similar difference was found in the allele frequency of +276G/T between two groups [X2=3.895, P=0.048]. The increased risk of CAD was associated with +276 TT genotype when compared to reference GG genotype [OR=5.158; 95% CI=1.016-26.182, P=0.048]. However, no similar difference was found in genotype and allele frequencies of SNP +45T/G between two groups. There was a CAD protective haplotype combination of +276 wild-type and +45 mutant-type allele [276G-45G] [OR=0.37, 95% CI=0.16-0.86, P=0.022] in the subject population
Conclusion: Our findings indicated that T allele of SNP +276G/T is more associated with the increased risk of CAD in subjects with T2DM. Also, a haplotype combination of +45G/+276G of these two SNPs has a protective effect on the risk of CAD